Aflatoxin M1 determination in Milk Samples
using LC-MS/MS

Aflatoxin B1 is the most common contaminant found in peanuts, cottonseed meal, corn, and other feed/food grains.
Aflatoxin B1 is absorbed in the gastrointestinal tract of the animals and is biotransformed primarily in the liver, by hydroxylation forming derivatives such as aflatoxin M1 which can be excreted through body fluids such as milk and in eggs also.
The aim of this work was to develop a fast and simple UHPLC-based method for the determination and accurate quantitation of Aflatoxin M1 regulated in food (EU Commission regulation (EC) Nº 1881/2006) liquid chromatography coupled with tandem mass spectrometry.
  • The milk samples were extracted with acetonitrile and deproteinated using centrifuge.
  • The blank milk samples were spiked with Aflatoxin M1 standard at three levels (LOQ, 5xLOQ, and 25xLOQ).
  • The average recovery of Aflatoxin M1 at the different levels 0.01, 0.05, and 0.25µg/kg varied between 79%-99%.
  • The method/curve is linear from 0.01µg/kg to 0.5µg/kg Aflatoxin M1 levels.
  • A sample of ERM® - BD284 whole milk powder sample was tested using this method.
This method on Aflatoxin M1 determination in milk samples has demonstrated good recovery and accurate results with the limit of detection to 0.01µg/kg.
Hunor Farkaš, Jog Raj, Jelena Leković, Robert Čepela, Jasna Bošnjak, Marko Vasiljević
PATENT CO. DOO., Vlade Cetkovica, Serbia

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