OBJECTIVE
OBJECTIVE
Mycotoxins are the biggest challenge for animal feed producers and can be remediated by various methods, but enzymes and microorganisms for deactivating mycotoxins are becoming increasingly popular.
![](https://mycotoxinsite.com/wp-content/uploads/2021/05/Objective.png)
METHODS
METHODS
In order to evaluate the microorganisms’ mycotoxin degradation capacity two flasks were prepared:
Control Flask
The Control Flask was prepared with culture medium and the mycotoxins DON, ZEN, AFB1, FB1, OTA and T-2 toxin at a final concentration of 0.2 ppm.
Test Flask
For the preparation of the Test Flask, on day 1 the microbial culture was inoculated in nutrient broth or mineral salt medium and incubated at 37˚C for 16-20 hours.
⇰ The next day, the mycotoxins DON, ZEN, AFB1, FB1, OTA and T-2 toxin were added to reach a final concentration of 0.2 ppm.
Both flasks were incubated at 37˚C and samples were taken at 0, 2, 6, 12 and 24 hours for analysis of degradation activity using LC-MS/MS.
![](https://mycotoxinsite.com/wp-content/uploads/2022/05/Mycotoxoxins.png)
RESULTS
RESULTS
The results showed that incubation of the Test Flask with Bacillus sp. for 2 hours resulted in:
- ⇰ Transformation of 26% of AFB1 to AFQ1
- ⇰ Transformation of 25% of OTA to OTA-α and OTA B (very low levels detected)
- ⇰ Transformation of 95% of ZEN to α-Zearalenol (only 39 ppb was detected and this level of α-Zearalenol does not have toxic effects).
![](https://mycotoxinsite.com/wp-content/uploads/2022/05/Poultry-mycotoxins.png)
Authors
J. Raj*, Z. Jakovčević, H. Farkaš, J. Bošnjak-Neumüller y M. Vasiljević
1PATENT CO, DOO., Vlade Ćetkovića 1A, 24 211, Mišićevo, Serbia
Corresponding author: [email protected]
References
MycoTWIN – MycoKey 2021 INTERNATIONAL CONFERENCE BARI, Italy, 9-12 November 2021