Jog Raj*, Hunor Farkaš, Zdenka Jakovcevic and Marko Vasiljevic
PATENT CO, DOO., Vlade Ćetkovića 1A, Mišićevo 24211, Serbia
*Corresponding author: [email protected]
⇰ The wheat and barley samples were collected from various regions of Serbia. These samples were analysed by LC-MS/MS triple quad (Agilent 6460 series) based multi-mycotoxin method for quantication of all mycotoxins present (Aflatoxin B1 (AFB1), B2, G1 and G2, Ochratoxin A (OTA), Zearalenone (ZEN), Deoxynivalenol (DON), Fumonisin B1 and B2 (FB1 & FB2), T-2 & HT-2 toxins) that are regulated in the EU in feed by EU Directive 2002/32/EC, 2006/576/EC and 2013/165/EU.
The results are summarised as shown below.
MYCOTOXINS DETECTED in Barley
In 2019, 97% of the barley samples were contaminated with one or more mycotoxins. In 2020, 23% of samples were contaminated with one or more mycotoxins. This data shows that barley harvested in 2020 is less contaminated with mycotoxins as compared to 2019.
Figure 1. Number (%) of mycotoxins detected in barley samples (2019 and 2020)
The mean contamination levels of OTA, ZEN, DON, FB1, HT-2 and T-2 were 8 ppb, 114 ppb, 3175 ppb, 51 ppb, 27 ppb and 19 ppb (Table 1). Aflatoxins were not detected in barley harvested in 2019. In 2020, ZEN, DON, HT-2, and T-2 with a level of 203 ppb, 1105 ppb, 29 ppb and 28 ppb were detected in barley samples. These results showed the co-occurrence of Fusarium mycotoxins and OTA in barley samples from Serbia, but the levels were higher in 2019 as compared to 2020 harvest.
Table 1. Mycotoxin contamination levels detected (ppb) in the barley samples from Serbia in 2019 and 2020
The data in Table 2 shows that in 2019 OTA in 15%, ZEN in 34%, DON in 67%, FB1 in 2%, HT-2 in 47% and T-2 in 21% samples were detected in barley samples. In 2020 ZEN in 4%, DON in 11%, HT-2 in 19 % and T-2 in 7% samples were detected in barley samples using LC-MS/MS.
Table 2. % positives of barley samples from Serbia in 2019 and 2020 barley harvest