Summary
OBJECTIVE
OBJECTIVE
The aim of this study was to determine the efficacy of a novel multicomponent mycotoxin detoxifying agent (MMDA) containing modified zeolite (Clinoptilolite), Bacillus subtilis, B. licheniformis, Saccharomyces cerevisiae cell walls and silymarin against the deleterious effects of aflatoxin B1 (AFB1) and ochratoxin A (OTA) in broiler chicks.
MATERIALS & METHODS
MATERIALS & METHODS
A total of 160 one-day-old Ross 308® broiler chicks were randomly allocated in four treatment groups for 42 days, with four replicates according to the following experimental design:
Group A: basal diet-
Group B: basal diet contaminated with 0.1 mg/kg AFB1 and 1 mg/kg OTA 
Group C: basal diet contaminated with 0.1 mg/kg AFB1, 1 mg/kg OTA, and MMDA at 1 g/kg feed
Grupo D: basal diet contaminated with 0.1 mg/kg AFB1, 1 mg/kg OTA, and MMDA at 3 g/kg feed
RESULTS
RESULTS
Results showed that ingested mycotoxins led to a significant (p ≤ 0.05) reduction in body weight and feed conversion from 25 days of age. Additionally, they:
Induced histopathological changes- Increased the pH of the intestinal content
- Altered the biochemical profile of birds with significantly (p ≤ 0.05) increased aspartate aminotransferase (AST) values (p ≤ 0.05)
On the other hand, supplementation with MMDA:
Significantly improved the feed conversion ratio (FCR) (p ≤ 0.05) during the second part of the study- Reduced biochemical alterations
- Reduced the pH in jejunal and ileal content
- Reduced E. coli counts in the caeca of birds (p ≤ 0.05)
Autores
Vasilios Tsiouris1, Panagiotis Tassis2, Jog Raj3, Tilemachos Mantzios1, Konstantinos Kiskinis1, Marko Vasiljevic3, Nikola Delic4, Evanthia Petridou5, Georgia D. Brellou6, Zoe Polizopoulou7, Nikolaos Mittas8 and Ioanna Georgopoulou1
1Unit of Avian Medicine, School of Veterinary Medicine, Aristotle University of Thessaloniki, Greece
2Clinic of Farm Animals, School of Veterinary Medicine, Aristotle University of Thessaloniki, Greece
3Patent Co, DOO., Misicevo, Serbia
4Institute for Animal Husbandry, Belgrade-Zemun, Serbia
5Laboratory of Microbiology and Infectious Diseases, School of Veterinary Medicine, Aristotle University of Thessaloniki, Greece
6Laboratory of Pathology, School of Veterinary Medicine, Aristotle University of Thessaloniki
7Diagnostic Laboratory, School of Veterinary Medicine, Aristotle University of Thessaloniki, Greece
8Department of Chemistry, School of Science, International Hellenic University, Greece
Referencias
Tsiouris, V.; Tassis, P.; Raj, J.; Mantzios, T.; Kiskinis, K.; Vasiljevi ́c, M.; Deli ́c, N.; Petridou, E.; Brellou, G.D.; Polizopoulou, Z.; et al. Investigation of a Novel Multicomponent Mycotoxin Detoxifying Agent in Amelioration of Mycotoxicosis Induced by Aflatoxin-B1 and Ochratoxin A in Broiler Chicks. Toxins 2021, 13, 367. https://doi. org/10.3390/toxins13 060367
Micotoxicosis prevention