Multiple mycotoxins detected in corn samples received from five continentsbetween October 2018 and March 2019

Mycotoxins are the biggest challenge for animal feed producers and therefore, regular monitoring of mycotoxins is necessary

Jog Raj, Hunor Farkaš, Zdenka Jakovčević, Jasna Bošnjak-Neumüller and Marko Vasiljević

PATENT CO., Mišićevo, Serbia


The aim of the present study was to screen corn samples for mycotoxins, received from different countries (five continents) between October 2018 and March 2019.


These corn samples were collected by our customers or distributors in different countries.

The corn samples were analysed by LC-MS/MS triple quad (Agilent 6460 series) based multi-mycotoxin method for quantitation of all mycotoxins (Aflatoxin B1 (AFB1), B2, G1 and G2, Ochratoxin A (OTA), Zearalenone (ZON), Deoxynivalenol (DON), FB1&B2, T-2 & HT-2 toxins) regulated in EU in feed by EU Directive 2002/32/EC, 2006/576/EC and 2013/165/EU:

  • ⇰ Aflatoxins: AFB1, AFB2, AFG1 y AFG2
  • ⇰ Ochratoxin A (OTA)
  • ⇰ Zearalenone (ZON)
  • ⇰ Deoxynivalenol (DON)
  • ⇰ FB1 & FB2
  • ⇰ Toxins T-2 & HT-2


  • 🔎 A total of 400 samples were received for analysis at PATENT CO.
  • 🔎 More than 95% of the samples were found contaminated with one or more mycotoxins


The summary of mycotoxins analysis as per continent is as below:


98 % of the corn samples received from India, Pakistan, Vietnam, Taiwan and Thailand were found contaminated with one or more mycotoxins (Figure 1).

Figure 1. Number (%) of mycotoxins per sample.

Table 1. Mycotoxins levels detected (ppb) in corn samples from Asia

DON, AFB1, FB1, FB2, T-2, OTA and ZON with an average of 300 ppb, 18 ppb, 1.701 ppb, 452 ppb, 19 ppb, 2 ppb and 81 ppb, respectively, were among the main mycotoxins detected (Table 1) in corn samples from Asia.

The median for FB1 was 1.019 ppb, FB2 was 258 ppb and for DON median was recorded at 367 ppb.

FB1 and FB2 were detected in the 94% of corn samples, while AFB1, ZON and DON, 72%, 26% and 10% of the samples, respectively (Figure 2).

Figure 2. Percentage of mycotoxins detected

These results demonstrate that Fusarium mycotoxins (FB1, FB2, DON, ZON) and Aflatoxins were detected in 98% of corn samples tested using LC-MS/MS.

Micotoxicosis prevention
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