MATERIALS & METHODS
The method is based on ‘dilute-and-shoot’ principle.A total of 113 samples were received for analysis at PATENT CO., Mišicevo, Serbia.
It involves two-step extraction and centrifugation of the extracts.
To compensate the matrix effects in electrospray ionisation, the extracts are mixed with 13C-labelled internal standards for each group of mycotoxins (U-[13C17]-Aflatoxin B1, U-[13C15]- Deoxynivalenol, U-[13C18]-Zearalenone, U-[13C20]-Ochratoxin A, U-[13C34]-Fumonisin B1, and U- [13C24]-T-2) before injection onto LC-MS/MS (Agilent 6460c LC-MS/MS).
Aflatoxin B1, Fumonisin B1 and B2, and HT-2 toxin were detected in 13%, 44%, 24% and 8% samples, respectively.
- Aflatoxin B1 ranging from 0.59 to 5,644 ppb
- HT-2 toxin ranging from 9 to 66 ppb
- Fumonisin B2 ranging from 53 to 2,540 ppb
- Fumonisin B1 ranging from 48 to 8,623 ppb
The aim of the present study was to screen maize samples received from Serbia and Bosnia and Herzegovina during August to November 2017.