Efficacy of a modified clinoptilolite-based adsorbent against the detrimental effects of Ochratoxin A in laying hens


The aim of this study was to evaluate the efficacy of modified clinoptilolite (Minazel Plus®) as a mycotoxin adsorbent for mitigating the effects of ochratoxin A (OTA) on the performance of laying hens and OTA-associated pathohistological changes, as well as the presence of OTA residue in their eggs.


Forty-eight laying hens (27 weeks old) were equally divided into six groups and allocated to the following experimental treatments for 7 weeks:

  • Control group: without feed additive
  • Grupo MZ: 0.2% of Minazel Plus®
  • Grupo E-I: 1 mg/kg OTA
  • Grupo E-II: 0,25 mg/kg OTA
  • Grupo E-III: 1 mg/kg OTA + 0.2% of Minazel Plus®
  • Grupo E-IV: 0.25 mg/kg OTA + 0.2% of Minazel Plus®


Overall, the addition of 0.2% Minazel Plus® in laying hens feed mitigated the harmful effects of OTA on target organs and reduced the presence of OTA residues in eggs.

The groups that received 0.2% of Minazel Plus® achieved better production results in terms of body weight, number of eggs, and feed consumption, compared to the other treatments.

The current findings confirm the efficacy of Minazel Plus® in preventing performance losses in laying hens exposed to OTA, as well as for improving the welfare and health of food producing animals.


Marko Vasiljević1, Darko Marinković2, Dragan Milićević3, Jelka Pleadin4, Srđan Stefanović3, Saša Trialović5, Jog Raj6, Branko Petrujkić7 y Jelena Nedejković Trialović7

1Faculty of Veterinary Medicine, University of Belgrade, Serbia
2Department of Pathology, Faculty of Veterinary Medicine, University of Belgrade, Serbia
3Institute of Meat Hygiene and Technology, Belgrade, Serbia
4Croatian Veterinary Institute, Laboratory for Analytical Chemistry, Zagreb, Croatia
5Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Belgrade, Serbia
6Patent Co, DOO., Mišićevo, Serbia
7Department of Animal Nutrition and Botany, Faculty of Veterinary Medicine, University of Belgrade, Serbia


Vasiljević, M.; Marinković, D.; Milićević, D.; Pleadin, J.; Stefanović, S.; Trialović, S.; Raj, J.; Petrujkić, B.; Trialović, J.N. Efficacy of a Modified Clinoptilolite Based Adsorbent in Reducing Detrimental Effects of Ochratoxin A in Laying Hens. Toxins 2021, 13, 469. https://doi.org/10.3390/toxins13070469

Micotoxicosis prevention
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